CD70 polyclonal antibody

• Background:

  CD70 is a type II transmembrane glycoprotein and a member of the tumor necrosis factor ligand superfamily (TNFSF), also known as CD27L and TNFSF7. It is normally expressed on medullary thymic epithelial cells. Its expression is induced on activated lymphoid cells (B cells, T cells, and NK cells) and dendritic cells. CD70 is a ligand for CD27, a co-stimulatory receptor that plays an important role in T cell activation and proliferation. CD70 overexpression has been reported in various tumors such as renal cell carcinoma, glioblastoma, and non-small cell lung carcinoma and it’s being actively pursued as a therapeutic target.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  CD70 antigen; CD27 ligand; CD27-L; Tumor necrosis factor ligand superfamily member 7; CD70; CD27L; CD27LG; TNFSF7

• Swiss Prot:

  P32970

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant protein of human CD70.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB: 1:2000~1:5000 IF: 1:100~1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 33 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD70 polyclonal antibody detects endogenous levels of CD70 protein.

• Applications Notes:

  Western blot (WB) analysis of CD70 polyclonal antibody at 1:2000 dilution Lane1:EC9706 whole cell lysate (30ug) Lane2:MCF-7 whole cell lysate (30ug) Lane3:CT-26 whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4