VPS34 polyclonal antibody

• Background:

  VPS34 (vacuolar protein sorting-associated protein 34), also known as VPT29, VPL7, PEP15 or END12, is a 875 amino acid peripheral membrane protein that localizes to the golgi apparatus. Belonging to the PI3/PI4-kinase family, VPS34 is involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. The VPS34 PI3-kinase complex II is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes. It has been suggested that VPS34 may also be involved in ethanol tolerance and cell wall integrity.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Phosphatidylinositol 3-kinase catalytic subunit type 3 (EC:2.7.1.137) ; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3

• Swiss Prot:

  Q8NEB9

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to Human VPS34.

• Conjugation:

  Unconjugated

• Related Pathways:

  Phosphoinosotode Signaling, Adherens Junction signaling

• Applications:

  WB IP

• Dilution:

  WB: 1:500~1:1000 IP: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 100 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  VPS34 (phospho-Ser249) polyclonal antibody detects endogenous levels of VPS34 protein only when phosphorylated at Ser249.

• Applications Notes:

  Western blot (WB) analysis of PIK3C3/VPS34 pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4