NCF2 polyclonal antibody

• Background:

  NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production) . Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) . Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  67 a neutrophil oxidase factor; Chronic granulomatous disease autosomal 2; NADPH oxidase activator 2; NCF-2; Ncf2; NCF2; Neutrophil cytosol factor 2; Neutrophil NADPH oxidase factor 2; NOXA2; p67-phox; p67phox

• Swiss Prot:

  P19878

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length Human NCF2.

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IF/ICC IP

• Dilution:

  WB: 1:500~1:2000 IHC: 1:50~1:200 IF/ICC: 1:50~1:200 IP: 1:20~1:100

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 68 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  NCF2 polyclonal antibody detects endogenous levels of NCF2 protein.

• Applications Notes:

  Western blot analysis of p67 phox expression in THP1 (A), mouse spleen (B) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4