Alpha Synuclein Antibody (Aggregate-Specific): RPE

• Background:

  Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer’s disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Covered by US patent family US11,098,108B2 (7).

• Description:

  Mouse Anti-Mouse Alpha Synuclein Aggregate Antibody Monoclonal IgG1

• Specifications:

  Binds a 3-dimensional fibrillar epitope including AA101-120, AA61-80

• Product Name Alternative:

  Alpha Synuclein Antibody, Non-A beta component of AD amyloid Antibody, Non-A4 component of amyloid precursor Antibody, NACP Antibody, SNCA Antibody, PARK1 Antibody, PARK 1 Antibody, alphaSYN Antibody, PARK 4 antibody, PARK4 antibody, Parkinson disease familial 1 antibody, Parkinson disease (autosomal dominant, Lewy body) 4 antibody, SYN antibody

• Synonyms:

  Alpha Synuclein Antibody (Aggregate-Specific), Clone 2F11: RPE

• UNSPSC:

  12352203

• Gene ID:

  20617

• Swiss Prot:

  O55042

• Accession Number:

  NP_001035916.1

• Cellular Locus:

  Cell Junction, Cytoplasm, Cytosol, Membrane, Nucleus, Synapse

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse

• Immunogen:

  Mouse alpha synuclein fibrils

• Target:

  Alpha Synuclein Aggregate Antibody

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  2F11

• Conjugation:

  RPE

• Validated Applications:

  WB, IHC, ICC/IF

• Purification:

  Protein G Purified

• Limit Of Detection:

  A 1:1000 dilution of SMC-617 was sufficient for detection of Alpha Synuclein aggregates in 15 µg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000). Application specific; optimal dilutions for assays should be determined by the user.

• Weight:

  0.1

• Buffer:

  95.64mM Phosphate, 2.48mM MES and 2mM EDTA

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. 7. Louwrier, A. US Patent family US11,098,108 B2

• CAS Number:

  9007-83-4