Ponceau S

• Description :

  Ponceau S (Acid Red 112) is a non-specific protein dye commonly used as a stain for Western blot. Ponceau S is used in an acidic aqueous solution that is compatible with antibody-antigen binding and dyes the proteins on the membrane red[1][2][3].

• Product Name Alternative :

  Acid Red 112

• UNSPSC :

  12171500

• Hazard Statement :

  H315, H319, H335

• Target :

  Fluorescent Dye

• Type :

  Dye Reagents

• Related Pathways :

  Others

• Field of Research :

  Others

• Assay Protocol :

  https://www.medchemexpress.com/ponceau-s.html

• Purity :

  98.0

• Solubility :

  DMSO : 100 mg/mL (ultrasonic) |H2O : 35.71 mg/mL (ultrasonic)

• Smiles :

  O=S(C1=C(O)C(/N=N/C2=CC=C(/N=N/C3=CC=C(S(=O)(O[Na])=O)C=C3)C=C2S(=O)(O[Na])=O)=C4C=CC(S(=O)(O[Na])=O)=CC4=C1)(O[Na])=O

• Molecular Formula :

  C22H12N4Na4O13S4

• Molecular Weight :

  760.57

• Precautions :

  H315, H319, H335

• References & Citations :

  [1]Sander H, et, al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem. 2019 Jun 15; 575: 44-53.|[2]Litovchick. Staining the Blot for Total Protein with Ponceau S. Cold Spring Harb Protoc. 2020 Mar 2; 2020 (3) : 098459.|[3]Romero-Calvo I, et al. Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal Biochem. 2010 Jun 15;401 (2) :318-20.

• Shipping Conditions :

  Room Temperature

• Storage Conditions :

  Store at room temperature, keep dry and cool

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [6226-79-5]