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Thymidylate Synthase Antibody

CAT: 0013-GTR18083281-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18083281-01Size:20 µg
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Description
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) . TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
UniProt
P04818
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this anti-Thymidylate Synthase antibody.
Clonality
Monoclonal
Clone
SPM453
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the anti-Thymidylate Synthase antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

UniProtKB · P04818

Thymidylate synthase

TYSY_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04818
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TYMS
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
TS, TSase
EC number
2.1.1.45
Processing
—
Secondary accessions
Q8WYK3, Q8WYK4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmMembraneMitochondrionMitochondrion inner membraneNucleus

Disease

Disease variantDyskeratosis congenita

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Molecular function

MethyltransferaseTransferase

Biological process

Nucleotide biosynthesis

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