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Gp100 Antibody / PMEL17

CAT: 0013-GTR18083266-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18083266-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma.The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments.
UniProt
P40967
Reactivity
Human
Immunogen
Extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.
Clonality
Monoclonal
Clone
SPM142
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the gp100 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

UniProtKB · P40967

Melanocyte protein PMEL

PMEL_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P40967
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PMEL
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B3KS57, B7Z6D7, Q12763, Q14448, Q14817, Q16565
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Cellular component

Endoplasmic reticulumEndosomeGolgi apparatusMembraneSecreted

Biological process

Melanin biosynthesis

Domain

RepeatSignalTransmembraneTransmembrane helix

Ligand

Sialic acid

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