Products for Research Use Only
RegisterLogin

EpCAM Antibody

CAT: 0013-GTR18083232-01Size: 20 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18083232-01Size:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
UniProt
P16422
Reactivity
Human
Immunogen
Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody. The epitope has been localized to amino acids 27-59.
Clonality
Monoclonal
Clone
SPM528
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the EpCAM antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR18317649-01EPCAM Biosimilar Antibody
GTR18299428-01EpCAM monoclonal antibody
GTR17699499Epcam ICD Antibody
GTR18280164EPCAM Conjugated Antibody
GTR17702468EpCAM/CD326 Antibody
GTR18044633-01EpCAM polyclonal antibody
GTR18406097-01EPCAM Biosimilar Antibody
GTR17858846EpCAM Monoclonal Antibody
GTR18579613EPCAM Biosimilar Antibody
GTR18317642-01EPCAM Biosimilar Antibody

Alternative Products

CatalogName
10R-E110aEpCAM antibody

Popular Products