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HSP27 Antibody

CAT: 0013-GTR18083217-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18083217-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . Hsp27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
UniProt
P04792
Reactivity
Human
Immunogen
Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
Clonality
Monoclonal
Clone
SPM252
Conjugation
Unconjugated
Field of Research
Epigenetics, Infectious Diseases, Neuroscience
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the HSP27 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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