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Fibronectin Antibody

CAT: 0013-GTR18083211-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18083211-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibronectin is a dimeric glycoprotein of 440kDa which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This mAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin.
UniProt
P02751
Reactivity
Human
Immunogen
Purified human fibronectin was used as the immunogen for this Fibronectin antibody.
Clonality
Monoclonal
Clone
SPM539
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 minutes.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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