SARS-CoV-2 surrogate Virus Neutralizing Test (sVNT) (spike protein/ ACE2 ligand binding assay) COVID-19 sVNT

• Description:

  Neutralizing antibodies develop in some patients to varying degrees following infection with SARS-CoV-2

• Reaction Volume:

  40 µL

• Target Antigen:

  Neutralizing antibodies

• Detection Method:

  Competitive ELISA

• Assay Principle:

  Quantification of neutralizing antibodies

• Assay Protocol:

  This is a competitive ELISA that measures COVID19 neutralizing antibodies in human sera and plasma that prevents the binding of viral spike protein to its ACE2 receptor target.

• Assay Performance Time:

  2.5 Hours

• Sample Type:

  Serum Plasma

• Precision:

  <10%, <10%

• Sensitivity:

  SARS-CoV-2 neutralizing antibodies

• Components:

  Coated microtiter plate, 96 wells Calibrators (250 uL) - 0%, 20%, 40%, 60%, 80%, 100% inhibition 10X wash buffer - 50mL Assay buffer - 50mL 1000X detection reagent - 150 ul TMB - 12mL TMB stop solution - 12mL Plate sealers - 3

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C

• Shelf Life:

  12 months