GCSF ADA (granulocyte colony stimulating factor) ELISA

GCSF ADA (granulocyte colony stimulating factor) ELISA

• Description: Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream
• CAS Number: 7732-18-5
• Reaction Volume: 15 µL
• Target Antigen: antibodies to GCSF
• Detection Method: Direct sandwich ELISA
• Assay Principle: Quantification of antibodies to G-CSF
• Assay Protocol: This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured
• Assay Performance Time: 2.5 Hours
• Sample Type: Serum Plasma
• Detection Range: 45 ng/mL -1440 ng/mL
• Precision: <20%, <20%
• Sensitivity: anti-GCSF antibodies
• Limit Of Detection: 45 ng/mL
• Components: Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 mL Calibrator (1.15mg/mL) 12 μl 10X wash buffer 50 mL Assay buffer 50 mL 1000X detection reagent 17 μl TMB 12 mL TMB stop solution
• Shipping Conditions: Blue Ice
• Storage Conditions: -20°C
• Shelf Life: 12 months