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Recombinant Mitochondrial Marker Antibody

CAT: 0013-GTR18082813-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082813-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors.
Reactivity
Human
Immunogen
A semi-purified mitochondria preparation was used as the immunogen for this recombinant Mitochondrial Marker antibody.
Clonality
Recombinant
Clone
MTC02/2860R
Conjugation
Unconjugated
Purification
Protein A affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Mitochondrial Marker antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Rabbit
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Rabbit IgG, kappa

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