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CD47 Antibody / IAP

CAT: 0013-GTR18082377-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082377-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
UniProt
Q08722
Reactivity
Human
Immunogen
Amino acids 18-135 from the human protein were used as the immunogen for the CD47 antibody.
Clonality
Monoclonal
Clone
CDLA47-1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation; Cell Biology
Purification
Protein G affinity chromatography
Dilution
Immunofluorescence/Immunocytochemistry: 1-2ug/ml, Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD47 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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