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CD34 Antibody

CAT: 0013-GTR18082370-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082370-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. CD34 is an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. Conversely, under other circumstances CD34 has been shown to act as molecular Teflon and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumens. Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration. [Wiki]
UniProt
P28906
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the CD34 antibody.
Clonality
Monoclonal
Clone
CDLA34-1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow Cytometry: 0.5-1ug/million cells in 0.1ml, Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Titering of the CD34 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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