Products for Research Use Only

HSP27 Antibody

CAT: 0013-GTR18082303-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082303-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HSP27 is induced by environmental stress and developmental changes. It is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. [RefSeq]
UniProt
P04792
Reactivity
Human
Immunogen
Recombinant human HSPB1 protein was used as the immunogen for the HSP27 antibody.
Clonality
Monoclonal
Clone
SRPR27
Conjugation
Unconjugated
Field of Research
Epigenetics, Infectious Diseases, Neuroscience
Purification
Protein G affinity chromatography
Dilution
Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the HSP27 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IF, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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