EMI-1 Antibody

CAT: 0013-GTR18082292-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18082292-01Size:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

UniProt

Q9UKT4

Reactivity

Human

Immunogen

A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Clonality

Monoclonal

Clone

FBXP5

Conjugation

Unconjugated

Field of Research

Epigenetics

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the EMI-1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2a, kappa

UniProtKB · Q9UKT4

F-box only protein 5

FBX5_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q9UKT4
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: FBXO5
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: B3KNX5, Q5TF47, Q8WV29, Q9UGC8

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell divisionMitosisUbl conjugation pathway

Cellular component

CytoplasmCytoskeletonNucleus

Ligand

Metal-bindingZinc

PTM

PhosphoproteinUbl conjugation

Domain

Zinc-finger

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