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CD45RA Antibody

CAT: 0013-GTR18082276-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082276-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD45RA is an isoform of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells.
UniProt
P08575
Reactivity
Human
Immunogen
Recombinant human protein was used as immunogen for this anti-CD45RA antibody.
Clonality
Monoclonal
Clone
CDLA45RA-1
Conjugation
Unconjugated
Field of Research
Epigenetics, Infectious Diseases
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 ug/ml, Immunohistology (FFPE) : 0.5-1.0ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Titering of the anti-CD45RA antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2a, kappa

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