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Cyclin D1 Antibody

CAT: 0013-GTR18082248-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082248-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t (11; 14) (q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative) . Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
UniProt
P24385
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the Cyclin D1 antibody.
Clonality
Monoclonal
Clone
BCLD1-1
Conjugation
Unconjugated
Field of Research
Cancer, DNA Damage, Epigenetics
Purification
Protein G affinity chromatography
Dilution
Flow Cytometry: 0.5-1ug/million cells in 0.1ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2a, kappa

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