PCNA Antibody

CAT: 0013-GTR18082211-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18082211-01Size:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Proliferating cell nuclear antigen is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. [UniProt]

UniProt

P12004

Reactivity

Human

Immunogen

Recombinant proliferating cell nuclear antigen was used as the immunogen for the PCNA antibody.

Clonality

Monoclonal

Clone

PM441-1

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the PCNA antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IHC-P, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2a, kappa

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