Vimentin Antibody

CAT: 0013-GTR18082210-02Size: 100 µgDry Ice: NoHazardous: No

1 / 1

CAT#:0013-GTR18082210-02Size:100 µg

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Description

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]

UniProt

P08670

Reactivity

Human

Immunogen

Full length human recombinant protein was used as the immunogen for the Vimentin antibody.

Clonality

Monoclonal

Clone

VIM66-1

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the Vimentin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

UniProtKB · P08670

Vimentin

VIME_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P08670
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: VIM
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: B0YJC2, D3DRU4, Q15867, Q15868, Q15869, Q548L2, Q6LER9, Q8N850, Q96ML2, Q9NTM3

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondPhosphoproteinS-nitrosylationUbl conjugation

Disease

CataractDisease variant

Cellular component

Cell membraneCell projectionCytoplasmCytoskeletonIntermediate filamentMembraneNucleus

Domain

Coiled coil

Biological process

Host-virus interaction

Related Products

Catalog	Name
GTR19010819-01	Vimentin
GTR19010352-01	Vimentin
GTR19020946-01	Vimentin
GTR19010815-01	Vimentin
GTR19010738-01	Vimentin
GTR19010297-01	Vimentin
GTR18290652-01	Vimentin monoclonal antibody
GTR18195551	Vimentin Polyclonal Antibody
GTR18195549	Vimentin Polyclonal Antibody
GTR18195364	Vimentin Polyclonal Antibody

Alternative Products

Catalog	Name
20C-CR2052G	Vimentin antibody
GWB-9CC09D	Vimentin, Antibody