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GLUT1 Antibody

CAT: 0013-GTR18082153-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082153-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 45-55kDa, which is identified as GLUT1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut1 to Glut12. Glut1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut1 is expressed at variable levels in many human tissues. Overexpression has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative) .
UniProt
P11166
Reactivity
Human
Immunogen
A portion of amino acids 203-305 from the human protein was used as the immunogen for this GLUT1 antibody.
Clonality
Monoclonal
Clone
GLUT1/2475
Conjugation
Unconjugated
Field of Research
Epigenetics
Purification
Protein G affinity chromatography
Dilution
ELISA: 1-2ug/ml for coating (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the GLUT1 antibody should be determined by the researcher.
Tested Applications
ELISA, FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2b, kappa

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