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Recombinant FOXA1 Antibody

CAT: 0013-GTR18081945-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081945-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate. [UniProt]
UniProt
P55317
Reactivity
Human, Rat
Immunogen
A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this recombinant FOXA1 antibody.
Clonality
Recombinant
Clone
RFOXA1/1515
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Flow cytometry: 1-2ug/million cells
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant FOXA1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

UniProtKB · P55317

Hepatocyte nuclear factor 3-alpha

FOXA1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P55317
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
FOXA1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
HNF-3-alpha, HNF-3A
EC number
—
Processing
—
Secondary accessions
B2R9H6, B7ZAP5, Q9H2A0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

ActivatorChromatin regulatorDevelopmental proteinDNA-bindingRepressor

Coding sequence diversity

Alternative splicing

Cellular component

Nucleus

PTM

Phosphoprotein

Biological process

TranscriptionTranscription regulation

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