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Recombinant CD44v9 Antibody

CAT: 0013-GTR18081907-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081907-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
UniProt
P16070
Reactivity
Human
Immunogen
An amino acid sequence from the variant 9 domain of CD44 was used as the immunogen for the recombinant CD44v9 antibody.
Clonality
Recombinant
Clone
RCD44v9/1459
Conjugation
Unconjugated
Field of Research
Epigenetics
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD44v9 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

UniProtKB · P16070

CD44 antigen

CD44_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P16070
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CD44
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A5YRN9, B6EAT9, D3DR12, D3DR13, O95370, P22511, Q04858, Q13419, Q13957, Q13958, Q13959, Q13960, Q13961, Q13967, Q13968, Q13980, Q15861, Q16064, Q16065, Q16066, Q16208, Q16522, Q86T72, Q86Z27, Q8N694, Q92493, Q96J24, Q9H5A5, Q9UC28, Q9UC29, Q9UC30, Q9UCB0, Q9UJ36
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Blood group antigenReceptor

Biological process

Cell adhesion

Cellular component

Cell membraneCell projectionMembraneSecreted

PTM

Disulfide bondGlycoproteinPhosphoproteinProteoglycan

Domain

SignalTransmembraneTransmembrane helix

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