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Rat EpCAM Antibody

CAT: 0013-GTR18081614-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18081614-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
UniProt
O55159
Reactivity
Mouse, Rat
Immunogen
Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.
Clonality
Monoclonal
Clone
Epcam/1158
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Unpurified high titer supernatant
Dilution
Immunohistochemistry (FFPE) : 1:50-1:100 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (3)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the rat EpCAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The epitopes of mAbs Epcam/1158 and Epcam/1159 monoclonal antibodies are different.3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Bioreactor concentrate with 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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