SHBG Antibody

CAT: 0013-GTR18081520-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18081520-01Size:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Recognizes a protein of 45kDa, identified as SHBG. It functions as an androgen transport protein, but may also be involved in receptor-mediated processes. Each dimer binds one molecule of steroid. It is specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. SHBG regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.

UniProt

P04278

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the SHBG antibody.

Clonality

Monoclonal

Clone

SHBG/245

Conjugation

Unconjugated

Field of Research

Epigenetics

Purification

Protein G affinity chromatography

Dilution

ELISA: order BSA/sodium azide-free format for coating, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the SHBG antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

ELISA, IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

UniProtKB · P04278

Sex hormone-binding globulin

SHBG_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P04278
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: SHBG
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: SHBG
EC number: —
Processing: Precursor
Secondary accessions: B0FWH4, B4DYU0, E9PGW1, F5H5Z8, I3L1N7, P14689, Q16616, Q3MIL0, Q6ISD2

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoprotein

Ligand

Lipid-bindingSteroid-binding

Domain

RepeatSignal

Cellular component

Secreted

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