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Cytochrome C Antibody

CAT: 0013-GTR18081480-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081480-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cytochrome C, or CYCS, is an electron carrier protein. The oxidized form of the CYCS heme group can accept an electron from the heme group of the Cytochrome C1 subunit of cytochrome reductase. CYCS then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. CYCS also plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of CYCS into the cytosol. Binding to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. [UniProt]
UniProt
P99999
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the Cytochrome C antibody.
Clonality
Monoclonal
Clone
CYCS/1010
Conjugation
Unconjugated
Field of Research
Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Cytochrome C antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2b, kappa

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