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MUC5AC Antibody

CAT: 0013-GTR18081431-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081431-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC) . This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.It has been found that treating the testing sample with Papain or mercaptoethanol will destroy the M1 epitope for the 2-11M1 antibody (Ref. 1; Cancer Research 46.8 (1986) : 3983-3989.)
UniProt
P98088
Reactivity
Human, Mouse
Immunogen
An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient was used as the immunogen for the MUC5AC antibody.
Clonality
Monoclonal
Clone
2-11M1
Conjugation
Unconjugated
Field of Research
Epigenetics
Purification
Protein G affinity chromatography
Dilution
ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format), Western blot: 1-2ug/ml, Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the MUC5AC antibody should be determined by the researcher.
Tested Applications
ELISA, FACS, IF, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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