Products for Research Use Only
RegisterLogin

MITF Antibody Cocktail

CAT: 0013-GTR18081414-01Size: 20 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18081414-01Size:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
UniProt
O75030
Reactivity
Human
Immunogen
An N-terminus fragment (D5) and recombinant human protein (MITF/915) were used as the immunogen for the MITF antibody cocktail.
Clonality
Monoclonal
Clone
D5 + MITF/915
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the MITF antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

UniProtKB · O75030

Microphthalmia-associated transcription factor

MITF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O75030
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MITF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B4DJL2, D3K197, E9PFN0, Q14841, Q9P2V0, Q9P2V1, Q9P2V2, Q9P2Y8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

ActivatorDevelopmental proteinDNA-binding

Disease

AlbinismDeafnessDisease variantMicrophthalmiaOsteopetrosisWaardenburg syndrome

Coding sequence diversity

Alternative splicing

Domain

Coiled coil

Cellular component

CytoplasmLysosomeMembraneNucleus

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Biological process

TranscriptionTranscription regulation

Related Products

CatalogName
GTR18574060MITF Antibody
GTR18574059MITF Antibody
GTR17919321-01MITF Antibody
GTR17926305-01MITF Antibody
GTR18591985-01MITF Antibody
GTR18233745-01MITF Antibody
GTR18192608-01MITF Antibody
GTR18192607-01MITF Antibody
GTR18192606-01MITF Antibody
GTR18152779MITF Antibody

Alternative Products

CatalogName
V2715-100UGMITF Antibody Cocktail
NBP2-66627Mouse Monoclonal MITF Antibody (C5/D5 Cocktail)
GWB-36C5ADMITF, Antibody
AF7641-01MITF Antibody

Popular Products