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CD99 Antibody

CAT: 0013-GTR18081406-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081406-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]
UniProt
P14209
Reactivity
Human, Rat
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.
Clonality
Monoclonal
Clone
HO36-1.1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
PEG precipitation
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Western blot: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD99 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgM, kappa

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