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Alpha Fetoprotein Antibody

CAT: 0013-GTR18081232-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081232-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop) . This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
UniProt
P02771
Reactivity
Human, Mouse
Immunogen
AFP purified from serum of a hepatoma patient was used as the immunogen for the Alpha Fetoprotein antibody.
Clonality
Monoclonal
Clone
C2
Conjugation
Unconjugated
Field of Research
Epigenetics
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Alpha Fetoprotein antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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Alternative Products

CatalogName
GWB-C60D62Alpha Fetoprotein Antibody
10R-11470Alpha-fetoprotein antibody
F4100-03PFetoprotein, Alpha (alpha Fetoprotein, Alpha, alpha-Fetoprotein, Alpha, alpha-1-Fetoprotein, Alpha, Afp, alpha-Fetoglobulin)
F4100-03RFetoprotein, Alpha (alpha Fetoprotein, Alpha, alpha-Fetoprotein, Alpha, alpha-1-Fetoprotein, Alpha, Afp, alpha-Fetoglobulin) (FITC)
136093Fetoprotein, Alpha (Alpha-fetoprotein, AFP, Alpha-1-fetoprotein, HPAFP)
F4100-01V-01Fetoprotein, Alpha (Alpha Fetoprotein, Alpha, Alpha-fetoprotein Precursor, AFP, Alpha-1-fetoprotein, Alpha-fetoglobulin, FETA, HPAFP)
303307Fetoprotein, Alpha (AFP, Alpha Fetoglobulin, Alpha Fetoprotein, Alpha Fetoprotein Precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, Alpha-fetoprotein, Hereditary Persistence of, Included, FETA, Hereditary Persistence of alpha Fetoprotein, HPAFP)

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