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P57Kip2 Antibody

CAT: 0013-GTR18081202-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081202-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.
UniProt
P49918
Reactivity
Human, Mouse
Immunogen
Recombinant human protein was used as the immunogen for the p57Kip2 antibody.
Clonality
Monoclonal
Clone
57P06
Conjugation
Unconjugated
Field of Research
Cancer, Epigenetics
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the p57Kip2 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2b, kappa

UniProtKB · P49918

Cyclin-dependent kinase inhibitor 1C

CDN1C_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P49918
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CDKN1C
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Cell cycle

Disease

Disease variantTumor suppressor

PTM

MethylationPhosphoprotein

Cellular component

Nucleus

Molecular function

Protein kinase inhibitor

Technical term

Proteomics identificationReference proteome

Domain

Repeat

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