Products for Research Use Only
RegisterLogin

CD8 Antibody (alpha)

CAT: 0013-GTR18081159-01Size: 20 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18081159-01Size:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.
UniProt
P01732
Reactivity
Human
Immunogen
A 13 amino acid peptide from the C-terminal cytoplasmic domain of the alpha chain was used as immunogen for this CD8 antibody.
Clonality
Monoclonal
Clone
C8/144B
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD8 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR18571765CD8A Antibody / CD8 alpha
GTR18089746-01CD8A Antibody / CD8 alpha
GTR18225355CD8A/CD8 Alpha Antibody
GTR17705254CD8 ALPHA Antibody
GTR17704317CD8 ALPHA Antibody
GTR17704314CD8 ALPHA Antibody
GTR17702950CD8 ALPHA Antibody
GTR18075041-01CD8 alpha Antibody
GTR18305161CD8 alpha Antibody
GTR17704891CD8 ALPHA Antibody

Alternative Products

CatalogName
GWB-E32F97ALPHA INTERFERON (IFN-alpha), Antibody
MBS8564691-01LT-alpha (Lymphotoxin-alpha) Antibody
MBS5680175-01Synuclein, alpha (alpha-Syn) Antibody
abx121829-01Alpha Adducin (alpha adducin) Antibody
abx134674-01alpha Tubulin (alpha tubulin) Antibody
MBS8523259-01IFN-alpha/betaR alpha Polyclonal Antibody
MBS565257-01alpha A/ alpha B-Crystallin polyclonal antibody
R-1379-50Anti-Internexin alpha (Alpha-Inx) Antibody
C-1377-50Anti-Alpha-internexin (Alpha-Inx) Antibody
V5187-100UGER alpha Antibody / Estrogen Receptor alpha

Popular Products