Products for Research Use Only
RegisterLogin

BrdU Antibody

CAT: 0013-GTR18081108-01Size: 20 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18081108-01Size:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Reactivity
All
Immunogen
BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.
Clonality
Monoclonal
Clone
BRD494
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1

Related Products

CatalogName
GTR18689055-01BrdU/5-BrdU Mouse Monoclonal Antibody
GTR18291319-01BrdU polyclonal antibody
GTR18294327-01BrdU polyclonal antibody
GTR18047753-01BrdU monoclonal antibody
GTR18074564-01BrdU (Bu20a) Antibody
GTR19346026BrdU Slides
GTR19161340-01Bromodeoxyuridine (BrdU)
GTR19345944Bromodeoxyuridine (BrdU)
GTR19168072-015-BrdU
GTR18716683Recombinant Bromodeoxyuridine Antibody / BrdU

Alternative Products

CatalogName
ANT-35174-100ugBrdU/5-BrdU Antibody
MBS1569321-01Anti-BrdU/5-BrdU Antibody
10R-2321BRDU antibody
GWB-3AD5A4BrdU, Antibody

Popular Products