Products for Research Use Only
RegisterLogin

Lambda Light Chain Antibody

CAT: 0013-GTR18080995-01Size: 20 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18080995-01Size:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
This antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Reactivity
Human
Immunogen
Purified human Ig was used as the immunogen for this Lambda Light Chain antibody.
Clonality
Monoclonal
Clone
LcN-2
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2a, kappa

Related Products

CatalogName
GTR18201204Lambda Light Chain Polyclonal Antibody
GTR18576705-01LAMBDA LIGHT CHAIN-FITC Antibody
GTR17853695Lambda Light Chain, Free Antibody
GTR18300314-01Lambda Light Chain polyclonal antibody
GTR18276159Lambda Light chain Conjugated Antibody
GTR18290809-01Lambda light chain monoclonal antibody
GTR18576709-01LAMBDA LIGHT CHAIN-CF488 Antibody
GTR18603160-01Lambda Light Chain Rabbit pAb
GTR18716885-01IGLV1-51 Antibody / Lambda Light Chain
GTR18302366-01Lambda Light chain Rabbit mAb Antibody

Alternative Products

CatalogName
L1185-11DLambda Light Chain

Popular Products