Products for Research Use Only

IgG Antibody

CAT: 0013-GTR18080983-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18080983-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 75kDa, identified as the gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Reactivity
Human
Immunogen
Purified human IgG heavy chain was used as the immunogen for this anti-IgG antibody.
Clonality
Monoclonal
Clone
ICO-97
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-IgG antibody to be titered up or down for optimal performance.
Tested Applications
ELISA, FACS, IF
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa