HSP27 Antibody

CAT: 0013-GTR18080975-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18080975-01Size:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

This antibody recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27) . HSP27 was recently found to be identical to the estrogen-induced 'p29' and '24K' protein. About 50% of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

UniProt

P04792

Reactivity

Human, Mouse, Rat

Immunogen

Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.

Clonality

Monoclonal

Clone

G3.1

Conjugation

Unconjugated

Field of Research

Epigenetics, Infectious Diseases, Neuroscience

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC-P, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

UniProtKB · P04792

Heat shock protein beta-1

HSPB1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P04792
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: HSPB1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: HspB1
EC number: —
Processing: —
Secondary accessions: B2R4N8, Q6FI47, Q96C20, Q96EI7, Q9UC31, Q9UC34, Q9UC35, Q9UC36

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationMethylationPhosphoprotein

Molecular function

Chaperone

Disease

Charcot-Marie-Tooth diseaseDisease variantNeurodegenerationNeuropathy

Cellular component

CytoplasmCytoskeletonNucleus

Biological process

Host-virus interactionStress response

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