CD34 Antibody

This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85% of angiosarcoma and Kaposiï¿1⁄2s sarcoma, but with a lower specificity.

Human

A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

QBEnd/10

Unconjugated

Immunology & Inflammation

Protein G purified antibody

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

For research use only.

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD34 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

FACS, IF, IHC-P

Mouse

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide