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Bcl-2 Antibody

CAT: 0013-GTR18080865-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18080865-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.
Reactivity
Human
Immunogen
A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.
Clonality
Monoclonal
Clone
124
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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