Arginase 1 Antibody / ARG1

• Description:

  Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1) . Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P05089

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant human protein fragment (within amino acids 1-200) was used as the immunogen for the Arginase 1 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  ARG1/8869R

• Type:

  Recombinant

• Applications:

  WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Arginase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Arginase 1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the Arginase 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasm

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free Arginase 1 antibody (clone ARG1/8869R) as confirmation of integrity and purity.