Liver Arginase Antibody / ARG1

• Description:

  Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1) . Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes, which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

• UniProt:

  P05089

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 1-200 from human ARG1 protein was used as the immunogen for the Liver Arginase antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  ARG1/9447

• Applications:

  IHC-P, WB

• Purity:

  Protein G affinity

• Format:

  Purified

• Limitations:

  This Liver Arginase antibody is available for research use only.

• Storage Conditions:

  Aliquot the Liver Arginase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Liver Arginase antibody should be determined by the researcher.

• Location:

  Cytoplasm

• Image Legend:

  Western blot testing of human liver tissue lysate with Liver Arginase antibody (clone ARG1/9447) . Predicted molecular weight ~35 kDa.