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HCG-alpha Antibody

CAT: 0800-V5174-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V5174-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human chorionic gonadotropin antibody (hCG) is a glycoprotein hormone synthesized in syncytiotrophoblastic cells of placenta and in certain trophoblastic tumors. The hormone-specific alpha chains have molecular weights of 13 kDa. HCG is found in moles and choriocarcinoma, chorionic components of germ cell tumors, and syncytiotrophoblast like cells in seminoma/dysgerminoma and embryonal carcinoma. In diagnostic pathology, hCG is a useful marker for classification of germ cell tumors, identification of extragonadal germ cell tumors.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P01215
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-116) from the human protein was used as the immunogen for the HCG-alpha antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RHCGa/6975
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the HCG-alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HCG-alpha antibody is available for research use only.
Storage Conditions
Aliquot the HCG-alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the HCG-alpha antibody should be determined by the researcher.
Location
Cytoplasm, Secreted
Image Legend
IHC staining of FFPE human placental tissue with HCG-alpha antibody (clone rHCGa/6975) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P01215

Glycoprotein hormones alpha chain

GLHA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01215
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CGA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoprotein

Molecular function

Hormone

Cellular component

Secreted

Domain

Signal

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