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SPARC Antibody / Osteonectin

CAT: 0800-V5109-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V5109-100UGSize:100 µg
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Description
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P09486
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the SPARC antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
OSTN/8528R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the SPARC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SPARC antibody is available for research use only.
Storage Conditions
Aliquot the SPARC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the SPARC antibody should be determined by the researcher.
Location
Secreted
Image Legend
IHC staining of FFPE human tonsil tissue with SPARC antibody (clone OSTN/8528R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P09486

SPARC

SPRC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09486
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SPARC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
D3DQH9, Q6IBK4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Basement membraneExtracellular matrixSecreted

Ligand

CalciumCopperMetal-binding

Disease

Disease variantOsteogenesis imperfecta

PTM

Disulfide bondGlycoprotein

Domain

Signal

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