PD1 Antibody / PDCD1

• Description:

  PDCD-1 (programmed cell death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. In addition, PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.

• Specifications:

  ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  Q15116

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment corresponding to the extracellular domain of human PDCD1 protein was used as the immunogen for the PD1 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  PDCD1/7276R

• Type:

  Recombinant

• Applications:

  ELISA, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the PD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PD1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the PD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the PD1 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell surface, Cytoplasm

• Image Legend:

  IHC staining of FFPE human tonsil tissue with PD1 (CD279) antibody (clone PDCD1/7276R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.