DES Antibody / Desmin

• Description:

  Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain Alpha-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P17661

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human desmin protein was used as the immunogen for the DES antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 k

• Clone:

  RDES/8846

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the DES antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This DES antibody is available for research use only.

• Storage Conditions:

  Aliquot the DES antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the DES antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasm

• Image Legend:

  IHC staining of FFPE human smooth muscle tissue with DES antibody (clone rDES/8846) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.