CD40 Antibody

• Description:

  CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

• Specifications:

  ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P25942

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment of human CD40 protein (around aa 1-200) was used as the immunogen for the CD40 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CD40/4940

• Applications:

  ELISA, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CD40 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD40 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD40 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD40 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell Surface, Secreted

• Image Legend:

  IHC staining of FFPE human placental tissue with CD40 antibody (clone CD40/4940) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.