CD45 Antibody

• Description :

  CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P08575

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A recombinant fragment of human PTPRC protein was used as the immunogen for the CD45 antibody.

• Clonality :

  Recombinant Monoclonal

• Isotype :

  IgG1 k

• Clone :

  RPTPRC/7275

• Type :

  Recombinant

• Applications :

  IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the CD45 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This CD45 antibody is available for research use only.

• Storage Conditions :

  Aliquot the CD45 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the CD45 antibody should be determined by the researcher.

• CAS Number :

  9000-83-3

• Location :

  Cell surface, Cytoplasm

• Image Legend :

  IHC staining of FFPE human tonsil tissue with CD45 antibody (clone rPTPRC/7275) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.