FCGR1A Antibody / CD64

• Description:

  CD64/FCGR1A is a surface glycoprotein with high affinity for monomeric IgG, is expressed constitutively on monocytes and macrophages, and can be induced in neutrophils subsequent to IFN-gamma stimulation. It plays a putative role in the initiation of cell-mediated cytotoxicity. Thus far, three genes encoding four distinct CD64 transcripts have been described. CD64/FCGR1A has been shown to associate with signal transducing subunit of the high affinity IgE receptor.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P12314

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the FCGR1A antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2 κ

• Clone:

  FCGR1A/7497

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the FCGR1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This FCGR1A antibody is available for research use only.

• Storage Conditions:

  Aliquot the FCGR1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the FCGR1A antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell Membrane

• Image Legend:

  IHC staining of FFPE human tonsil tissue with FCGR1A antibody (clone FCGR1A/7497) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.