Recombinant CD48 Antibody

• Description:

  Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI) -anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

• UniProt:

  P09326

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein sequence (within amino acids 59-178) from the human protein was used as the immunogen for the recombinant CD48 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  CD48/8360R

• Type:

  Recombinant

• Applications:

  IHC-P, WB

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant CD48 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant CD48 antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant CD48 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant CD48 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell surface

• Image Legend:

  Western blot testing of human Raji cell lysate with recombinant CD48 antibody. Expected molecular weight: 28-50 kDa depending on glycosylation level.