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SMAD4 Antibody

CAT: 0800-V4568-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V4568-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SMAD4 is implicated as a tumor suppressor since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80% of cases of pancreatic adenocarcinoma, but rarely in endometrial (0%), colorectal (0%), ovarian (3%), lung (0%), breast (2%) adenocarcinomas, and malignant melanoma (4%) . SMAD4is an important marker for confirming a diagnosis of pancreatic adenocarcinoma.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q13485
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 400-552) from the human protein was used as the immunogen for the SMAD4 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
SMAD4/7905R
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the SMAD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMAD4 antibody is available for research use only.
Storage Conditions
Aliquot the SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the SMAD4 antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
IHC staining of FFPE human salivary gland tissue with SMAD4 / DPC4 antibody (clone SMAD4/7905R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · Q13485

SMAD family member 4

SMAD4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13485
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SMAD4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SMAD 4, hSMAD4
EC number
—
Processing
—
Secondary accessions
A8K405
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Cellular component

CytoplasmNucleus

Disease

Disease variant

Molecular function

DNA-binding

Ligand

Metal-bindingZinc

Biological process

TranscriptionTranscription regulation

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